Leonid Mirny

MirnyLeonid Mirny

Associate Professor of Health Sciences and Technology
Associate Professor of Physics
Massachusetts Institute of Technology

Genome in 3D

DNA of the human genome is two meters long and is folded into a structure that fits in a cell nucleus. Recently developed Chromosome Conformation Capture technique (Hi-C) provides comprehensive information about spatial genome organization and its changes during the cell cycle. I will present our analysis of Hi-C data and polymer modeling of chromosome organization. In interphase, chromosome organization is characterized by multi-level domain architecture, which correlates with sequence features and local chromatin states. In metaphase, the organization is homogeneous, common to all chromosomes, and consistent among cell types, suggesting a general principle of metaphase chromosome structure. Using polymer simulations we build 3D models of metaphase chromosomes and suggest mechanisms of chromosome folding.