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Confocal Microscopy

The confocal microscope uses lasers as light source to scan samples that have been stained with fluorescent dyes. Our confocal microscope is equipped with an argon laser and a krypton laser. It is fitted with several lenses of different magnifications, including both dry and oil lenses.

This instrument is used in nearly all fields of science because of its ability to quickly obtain images without having to physically slice the specimen. When acquiring images, the confocal microscope can take single, 2-dimensional images as well as 'stacks,' or a series of images taken at incremental z-steps. These 'stacks' can be compiled into a 3-dimensional image and transformed it into a movie. Moreover, the images acquired can be edited to highlight only areas of interest. For example, it may show blood vessels of an alveoli but not the rest of lung tissue.

Microscope and Microscopy Images
Several images acquired with the microscope, pictures of the microscope itself and the facility are available below.

Random image 1
Random image 2
Random image 3
Cancer cells
Gingivitis growth
Pericytes in fat tissue

The Confocal Microscope
The Confocal Microscope
The Core Facility
The Lab (308A)
The Lab (308A)
The Lab (G24)
The Lab (G24)
Karen, Dan, and Melissa
Boston Skyline from Rick's office
Biomedical Imaging Laboratory  ||  Physiology  ||  Countway Library  ||  Harvard School of Public Health 
Biomedical Imaging Laboratory
Harvard School of Public Health
665 Huntington Ave, Boston, MA, USA  02115
imglab@hsphsun2.harvard.edu
(617) 432-2243 phone (617) 432-3468 fax

© 2001, The Harvard School of Public Health
Last update:  August 20, 2001