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David Kasahara

Research Associate

Department of Environmental Health

Department of Environmental Health

665 Huntington Avenue
Building 1, Room 319
Boston, Massachusetts 02115
Phone: 617.432.0989
Fax: 617.432.3468

Education

June 2010: Intensive Trainning in Translational Medicine (ITTM), Harvard Medical School

2008-2010: Research Fellow, Harvard School of Public Health

2005-2008: Post-doctoral Research Associate. University of Vermont

2001-2005: ScD, School of Medicine, University of Sao Paulo

1997-2000: MSc, School of Medicine, University of Sao Paulo

1992-1996: Pharmacy and Biochemistry, Faculty of Pharmaceutical Sciences, University of Sao Paulo

Research Interest

I am current working as Research Associate in Stephanie Shore’s Lab in several projects aimed primary in elucidate the mechanism(s) of asthma induced by obesity. In this lab I have two major interest: (1) role of adipokyne (mainly adiponectin) in ozone induced inflammation and (2) Rho Kinase and airway hyperresponsiveness.

I have been working with animal model of asthma (and other pulmonary diseases) and enviroment toxycology since 1997 with a major goal in becaming expert in pulmonary toxycology. For that, I worked with b2-adrenergic receptor agonist during my Masters and Doctoral degree in asthma model in guinea-pig and mouse. In 2005, I learned more about cigarette smoking with focus in acrolein and reactive volatile aldehydes. And since 2008, here at HSPH, I am learning more about pulmonary toxicology by working with ozone model of pulmonary inflammation and AHR.

 

Additional Information

  • Outstanding Service Award – HSPH Postdoctoral Association (2011)
  • American Thoracic Society Travel Award – ATS Assembly on Environmental and Occupational Health (2010)
  • National Postdoctoral Association – Nominating Committee (2011)
  • National Postdoctoral Association – Resource Development Committee (member) (2010-current)
  • Voting Member of HSPH Post-doctoral Association (2008-2009)
  • HSPH PDA – New Venture Chair (2010-2011)
  • The Journal of Immunology – reviewer
  • American Journal of Physiology – reviewer
  • Member of American Thoracic Society (since 2007)
  • Member of National Postdoctoral Association (since 2009)

Publications

 

  1. Kasahara DI, Kim HY, Williams AS, Verbout NG, Tran J, Si H, Wurmbrand AP, Jastrab J, Hug C, Umetsu DT, Shore SA. Pulmonary Inflammation Induced by Subacute Ozone Is Augmented in Adiponectin-Deficient Mice: Role of IL-17A. J Immunol. 2012 May 1;188(9):4558-67.
  2. Hizume DC, Toledo AC, Moriya HT, Saraiva-Romanholo BM, Almeida FM, Arantes-Costa FM, Vieira RP, Dolhnikoff M, Kasahara DI, Martins MA. Cigarette smoke dissociates inflammation and lung remodeling in OVA-sensitized and challenged mice. Respir Physiol Neurobiol. 2012 Mar 15;181(2):167-176.
  3. Shore SA, Williams ES, Chen L, Benedito LA, Kasahara DI, Zhu M. Impact of aging on pulmonary responses to acute ozone exposure in mice: role of TNFR1. Inhal Toxicol. 2011 Dec;23(14):878-88.
  4. Hristova M, Spiess PC, Kasahara DI, Randall MJ, Deng B, van der Vliet A. The Tobacco Smoke Component Acrolein Suppresses Innate Macrophage Responses by Direct Alkylation of c-Jun-N-terminal Kinase. Am J Respir Cell Mol Biol. 2011 Jul 21. PubMed PMID: 21778411.
  5. Lanças T, Kasahara DI, Gross JL, Pires-Neto RC, Deheinzelin D, Mauad T, Negri EM, Dolhnikoff M. Cholinergic hyperresponsiveness of peripheral lung  parenchyma in chronic obstructive pulmonary disease. Respiration. 2011;82(2):177-84.
  6. Zhu M, Liu PY, Kasahara DI, Williams AS, Verbout NG, Halayko AJ, Fedulov A,Shoji T, Williams ES, Noma K, Shore SA, Liao JK. Role of Rho kinase isoforms in murine allergic airway responses. Eur Respir J. 2011 Oct;38(4):841-50.
  7. Olson N, Kasahara DI, Hristova M, Bernstein R, Janssen-Heininger Y, van der Vliet A. Modulation of NF-?B and hypoxia-inducible factor–1 by S-nitrosoglutathione does not alter allergic airway inflammation in mice. Am J Respir Cell Mol Biol. 2011 Jun;44(6):813-23.
  8. Berntsen P, Park CY, Rothen-Rutishauser B, Tsuda A, Sager TM, Molina RM, Donaghey TC, Alencar AM, Kasahara DI, Ericsson T, Millet EJ, Swenson J, Tschumperlin DJ, Butler JP, Brain JD, Fredberg JJ, Gehr P, Zhou EH. Biomechanical effects of environmental and engineered particles on human airway smooth muscle cells. J R Soc Interface. 2010 Jun 6;7 Suppl 3:S331-40.
  9. Zhu M, Hug C, Kasahara DI, Johnston RA, Williams AS, Verbout NG, Si H, Jastrab J, Srivastava A, Williams ES, Ranscht B, Shore SA. Impact of adiponectin deficiency on pulmonary responses to acute ozone exposure in mice. Am J Resp Cell and Mol Biol, accept for publication 2009 Nov 9.
  10. Shore SA, Lang JE, Kasahara DI, Lu FL, Verbout NG, Si H, Williams ES, Terry RD, Lee A, Johnston RA. Pulmonary responses to subacute ozone exposure in obese versus lean mice. J Appl Physiol. 2009 Sep 10;107(5):1445-52. Epub 2009 Sep 10. PubMed PMID: 19745193.
  11. Boots AW, Hristova M, Kasahara DI, Haenen GR, Bast A, van der Vliet A. ATP-mediated activation of the NADPH oxidase DUOX1 mediates airway epithelial responses to bacterial stimuli. J Biol Chem. 2009 Jun 26;284(26):17858-67. Epub 2009 Apr 21. PubMed PMID: 19386603; PubMed Central PMCID: PMC2719424.
  12. Olson N, Greul AK, Hristova M, Bove PF, Kasahara DI, van der Vliet A. Nitric oxide and airway epithelial barrier function: regulation of tight junction proteins and epithelial permeability. Arch Biochem Biophys. 2009 Apr 15;484(2):205-13. Epub 2008 Dec 10. PubMed PMID: 19100237; PubMed Central PMCID: PMC2753865.
  13. Ruiz Schütz VC, Drewiacki T, Nakashima AS, Arantes-Costa FM, Prado CM, Kasahara DI, Leick-Maldonado EA, Martins MA, Tibério IF. Oral tolerance attenuates airway inflammation and remodeling in a model of chronic pulmonary allergic inflammation. Respir Physiol Neurobiol. 2009 Jan 1;165(1):13-21. Epub 2008 Sep 27. PubMed PMID: 18930843.
  14. Ckless K, Lampert A, Reiss J, Kasahara D, Poynter ME, Irvin CG, Lundblad LK, Norton R, van der Vliet A, Janssen-Heininger YM. Inhibition of arginase activity enhances inflammation in mice with allergic airway disease, in association with increases in protein S-nitrosylation and tyrosine nitration. J Immunol. 2008 Sep  15;181(6):4255-64. PubMed PMID: 18768883.
  15. Mauad T, Rivero DH, de Oliveira RC, Lichtenfels AJ, Guimarães ET, de Andre PA, Kasahara DI, Bueno HM, Saldiva PH. Chronic exposure to ambient levels of urban particles affects mouse lung development. Am J Respir Crit Care Med. 2008 Oct 1;178(7):721-8. Epub 2008 Jul 2. PubMed PMID: 18596224; PubMed Central PMCID: PMC2556454.
  16. Kasahara DI, Poynter ME, Othman Z, Hemenway D, van der Vliet A. Acrolein inhalation suppresses lipopolysaccharide-induced inflammatory cytokine production but does not affect acute airways neutrophilia. J Immunol. 2008 Jul 1;181(1):736-45. PMID: 18566440
  17. Nakashima AS, Prado CM, Lanças T, Ruiz VC, Kasahara DI, Leick-Maldonado EA, Dolhnikoff M, Martins MA, Tibério IF. Oral tolerance attenuates changes in in vitro lung tissue mechanics and extracellular matrix remodeling induced by chronic allergic inflammation in guinea pigs. J Appl Physiol. 2008 Jun;104(6):1778-85. PMID: 18388250
  18. Prado CM, Leick-Maldonado EA, Miyamoto L, Yano LM, Kasahara DI, Martins MA, Tibério IF. Capsaicin-sensitive nerves and neurokinins modulate non-neuronal nNOS expression in lung. Respir Physiol Neurobiol. 2008 Jan 1;160(1):37-44. PMID: 17897889
  19. Portela CP, Leick-Maldonado EA, Kasahara DI, Prado CM, Calvo-Tibério IF, Martins MA, Palermo-Neto J. Effects of stress and neuropeptides on airway responses in ovalbumin-sensitized rats. Neuroimmunomodulation. 2007;14(2):105-11. PMID: 17785991
  20. Hizume DC, Rivero DH, Kasahara DI, Leme AS, Arantes-Costa FM, Olivo CR, Sales P, Dolhnikoff M, Martins MA. Effects of positive end-expiratory pressure in an experimental model of acute myocardial infarct in wistar rats. Shock. 2007 May;27(5):584-9. PMID: 17438466
  21. Lanças T, Kasahara DI, Prado CM, Tibério IF, Martins MA, Dolhnikoff M. Comparison of early and late responses to antigen of sensitized guinea pig parenchymal lung strips. J Appl Physiol. 2006 May;100(5):1610-6. PMID: 16410372
  22. Fló C, Lopes FD, Kasahara DI, Silva AC, Jesus RC, Rivero DH, Saldiva PH, Martins MA, Jacob-Filho W. Effects of exercise training on papain-induced pulmonary emphysema in Wistar rats. J Appl Physiol. 2006 Jan;100(1):281-5. PMID: 16141386
  23. Prado CM, Leick-Maldonado EA, Kasahara DI, Capelozzi VL, Martins MA, Tibério IF. Effects of acute and chronic nitric oxide inhibition in an experimental model of chronic pulmonary allergic inflammation in guinea pigs. Am J Physiol Lung Cell Mol Physiol. 2005 Oct;289(4):L677-83. PMID: 15937069
  24. Kasahara DI, Perini A, Lopes FD, Arantes-Costa FM, Martins MA, Nunes MP. Effect of salbutamol on pulmonary responsiveness in chronic pulmonary allergic inflammation in guinea pigs. Braz J Med Biol Res. 2005 May;38(5):723-30. PMID: 15917953
  25. Prado CM, Leick-Maldonado EA, Arata V, Kasahara DI, Martins MA, Tibério IF. Neurokinins and inflammatory cell iNOS expression in guinea pigs with chronic allergic airway inflammation. Am J Physiol Lung Cell Mol Physiol. 2005 Apr;288(4):L741-8. PMID: 15579630
  26. Medeiros N Jr, Rivero DH, Kasahara DI, Saiki M, Godleski JJ, Koutrakis P, Capelozzi VL, Saldiva PH, Antonangelo L. Acute pulmonary and hematological effects of two types of particle surrogates are influenced by their elemental composition.Environ Res. 2004 May;95(1):62-70. PMID: 15068931
  27. Bussamra MH, da Silva Filho LV, Kasahara DI, Cukier A, Rodrigues JC, Stelmach R. Educational program for smoke-induced respiratory diseases in Brazilian Amazon:assessing knowledge about asthma. J Asthma. 2004 Feb;41(1):35-41. PMID: 15046376
  28. Leick-Maldonado EA, Kay FU, Leonhardt MC, Kasahara DI, Prado CM, Fernandes FT, Martins MA, Tibério IF. Comparison of glucocorticoid and cysteinyl leukotriene receptor antagonisttreatments in an experimental model of chronic airway inflammation in guinea-pigs. Clin Exp Allergy. 2004 Jan;34(1):145-52. PMID: 14720275
  29. Tibério IF, Leick-Maldonado EA, Miyahara L, Kasahara DI, Spilborghs GM, Martins MA, Saldiva PH. Effects of neurokinins on airway and alveolar eosinophil recruitment.Exp Lung Res. 2003 Apr-May;29(3):165-77. PMID: 12637228
  30. Leme AS, Kasahara DI, Nunes MP, Martins MA, Vieira JE. Exhaled nitric oxide collected with two different mouth pieces: a study in asthmatic patients. Braz J Med Biol Res. 2002 Oct;35(10):1133-7. PMID: 12424484
  31. Vieira JE, Cukier A, Stelmach R, Kasahara DI, Gannam S, Warth M. Comparison of knowledge on asthma: doctors completing internal medicine residency and doctors completing medical school. Sao Paulo Med J. 2001 May 3;119(3):101-4. PMID: 11391451