Dieter A. Wolf, M.D.
Professor, Signal Transduction Program
Director, NCI Cancer Center Proteomics Facility 

Burnham Institute for Medical Research

10901 North Torrey Pines Road
La Jolla, CA 92037
Tel 858.646.3117
Fax 858.646.3194
dwolf@burnham.org

http://www.burnham.org/labs/wolf/

We study posttranscriptional mechanisms of cellular control in the fission yeast Schizosaccharomyces pombe and in mammalian cancer cells. Our work focusses on three major areas:

1. Function and control of fission yeast cullin/RING ubiquitin ligases (CRLs)
2. Mechanisms controlling mRNA translation in fission yeast
3. The role of ubiquitin-dependent proteolysis of tumor suppressors in prostate cancer 

MD, 1992, University of Munich, Germany

Schmidt, M.W., Houseman, A., Ivanov, A.R., Wolf, D.A. (2007) Comparative proteomic and transcriptomic profiling of the fission yeast Schizosaccharomyces pombe. Molecular Systems Biology, 3:79

Zhou, C., Arslan, F., Wee, S., Kirshnan, S., Ivanov, A.R., Oliva, A., Leatherwood, J., Wolf, D.A. (2005) PCI proteins eIF3e and eIF3m define distinct translation initiation factor 3 complexes. BMC Biology, 3:14

Wee, S., Geyer, R., Toda, T., Wolf, D.A. (2005) CSN facilitates cullin/RING ubiquitin ligase function by counteracting autocatalytic adapter instability. Nat. Cell Biol., 7, 387-391

Doud, M.K., Schmidt, M.W., Hines, D., Naumann. C., Kocourek, A., Kashani-Poor, N., Zeidler, R., Wolf, D.A. (2004) Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis. BMC Genomics, 5:25

Wolf, D.A., Zhou, C., Wee, S. (2003) The COP9 signalosome: An assembly and maintenance platform for cullin ubiquitin ligases? Nat. Cell Biol. 2003, 5: 1029-1033

Schmidt, M., Jain, A., Wolf, D.A. (2003) Multidimensional proteomic analysis of proteolytic pathways involved in cell cycle control. In: Cell Cycle Checkpoint Control Protocols. Lieberman H. B. ed. New York: Humana Press Vol. 241: 235-245

Geyer, R., Wee, S., Anderson, S., Yates J.R.III, Wolf, D.A. (2003) BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. Molecular Cell 12, 783-790

Wolf, D.A., Geyer, R. (2003) Dynamic release of Cdc34 from SCF: The hand that rocks the cradle. Cell 114, 532-533

Zhou, C.; Wee, S.; Rhee, E.; Naumann, M.; Dubiel, W.; Wolf, D.A. (2003) Fission yeast COP9/signalosome suppresses cullin activity through recruitment of the deubiquitylating enzyme Ubp12p. Molecular Cell 11, 927-938

Wee, S., Hetfeld, B., Dubiel, W., Wolf, D.A. (2002) Conservation of the COP9/signalosome in budding yeast. BMC Genetics 3:15

Lu, L., Schulz, H., Wolf, D.A. (2002) The F-box protein SKP2 mediates androgen control of p27 stability in LNCaP human prostate cancer cells. BMC Cell Biology 3:22

Seibert, V., Prohl, C., Schoultz, I., Rhee, E., Lopez, R., Abderazzaq, K., Zhou, C., Wolf, D.A. (2002) Combinatorial diversity of fission yeast SCF ubiquitin ligases by homo- and heterooligomeric assemblies of the F-box proteins Pop1p and Pop2p. BMC Biochemistry 3:22

Zhou, C.; Seibert, V.; Geyer, R.; Rhee, E.; Lyapina, S.; Cope, G.; Deshaies, R.J.; Wolf, D.A. (2001) The fission yeast COP9/signalosome is involved in cullin modification by ubiquitin-related Ned8p. BMC Biochemistry 2:7

Lyapina, S.; Cope, G.; Shevchenko, A.; Serino, G.; Tsuge, T.; Zhou, C.; Wolf, D. A.; Wei, N.; Shevchenko, A.; Deshaies, R. J. (2001). Promotion of NEDD8-CUL1 conjugate cleavage by COP9 signalosome. Science 292, 1382-1385