Bioinformatics Tools

Bioinformatics Tools

                    
Plasmid Reannealing Temp. (Tra) Calculator

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This Excel spreadsheet will allow you to calculate the highest re-annealing temperature for your target DNA based on calculations given by Wu et. al. (Nuc. Acid Res. (33) e110), “A novel PCR strategy for high-efficiency automated site-directed mutagenesis”.