Our lab studies the molecular mechanisms that underlie the aging process, using both mammalian cell culture and the nematode worm C. elegans, in order to develop novel therapies to treat age-related pathologies.
By tagging proteins with fluorescent peptides we can visualize their localization and abundance in cells as the worms age. Here, AMPK (Green) is active in the intestinal nuclei, while CRTC-1 (Red) is inactive and sequestered to the cytosol.
Alternate fluorescent tagged proteins can be monitored and imaged live in different tissues in C. elegans
Using a dye that binds specifically to DNA, we can visualize each of the nuclei throughout the cells of the animals
C. elegans have approximately 1000 cells, 302 of which are neurons whose network has been fully mapped.