Techniques
We use a variety of techniques to measure diverse outcomes. Please contact us for more information about any. In addition, at the bottom of this page is a list of links to protocols and resources available on-line.
Genetically engineered mouse lines
TNFR1-deficient
TNFR1/RelA-double deficient
Triple mutants deficient in TNFR1, TNFR2, and IL-1RI
IL-6-deficient
Tet-inducible Cre in alveolar epithelial cells
Tet-inducible deletion of STAT3 in alveolar epithelial cells
MLE-15, A549, RAW, MHS, 3T3, CHO, HEK293, HEK293T
E. coli, S. pneumoniae, K. pneumoniae, S. aureus
tandem binding sites for STAT1, STAT3, or NF-κB
deletion series for the mouse IL6 promoter
transgenes for firefly luciferase, Renilla luciferase, or EGFP
constitutive promoters including SV40, CMV-IE, and ROSA26
transgenes including STAT3, STAT3C, RelA, and p50
Neutrophil recruitment
Lung mechanics (FlexiVent)
Spontaneous ventilation (Buxco)
Edema
Morphometry
Lung transfection (transthoracic electroporation)
Bacterial infections
Bacterial burden
Cell collection
DNA, RNA, and protein collection
Cell culture, primary and cell lines
Flow cytometry
Scanning cytometry
Microscopy, including light, epifluorescent, confocal, SEM, and TEM
Molecular biology and biochemistry
DNA, RNA, and protein purification
PCR, RT-PCR, and quantitative real-time RT-PCR
Genotyping
Northern blotting
siRNA
1-dimensional and 2-dimensional gel electrophoresis
EMSA
ELISA
immunoblots
immunoprecipitation
subcellular fractionation
sub-cloning
fusion proteins
transient transfection
NCBI provides multiple interactive databases, particularly useful for sequence analyses.
Jackson Labs makes many geneticaly engineered strains of mice available.
The Trans-NIH Mouse Initiatives site provides useful information about knockout mice.
The International Gene Trap Consortium site provides a searchable database of ES cell lines with genes trapped by insertional mutagenesis.
ATCC makes many cell lines and microbes available.
Protocol Online has a variety of protocols available for free.
Genomatix provides a variety of software, some free, for promoter analyses.
ExPASy Life Sciences Directory lists many useful links to databases, software, and web sites.
