Mechanistic and Biomolecular Studies

Nanoparticle–nanoparticle and nanoparticle-cells interactions have recently gained attention as they directly impact the cellular uptake and fate of nanoparticles. More specifically, the adsorbed proteins on nanoparticle surfaces and the formation of the so-called protein corona have a key role in these interactions, as they determine the nanoparticle agglomeration state, as well as their fate and transport in a biological media (mobility, settling, etc.). In our center we study the transport of nanoparticles in physiological media and physiological systems. We study the hydrodynamic diameter and effective density of the agglomerates that are formed when nanoparticles exposed to physiological fluids. We employ AFM to investigate directly the interactions of the nanoparticles with cells.

Relevant publications:

  1. J. M. Cohen, R. Derk, L. Wang, J. Godleski, L. Kobzik, J. Brain and P. Demokritou. “Tracking translocation of industrially relevant engineered nanomaterials (ENMs) across alveolar epithelial monolayers in vitro”. Nanotoxicology in press, DOI: 10.3109/17435390.2013.879612 (2014). link
  2. G. Pyrgiotakis, C. O. Blattmann, S. E. Pratsinis, P. Demokritou. “Nanoparticle-Nanoparticle interactions in biological media by Atomic Force Microscopy”. Langmuir 29, 11385–11395 (2013). link
  3. J. Cohen, G. DeLoid, G. Pyrgiotakis and P. Demokritou. “Interactions of engineered nanomaterials in physiological media and implications for in vitro dosimetry”. Nanotoxicology 7, 417-431 (2013). link
  4. C. Tsai, M. Echevarría-Vega, G. A. Sotiriou, C. Santeufemio, D. Schmidt, P. Demokritou and M. Ellenbecker. “Evaluation of environmental filtration control of engineered nanoparticles using the Harvard Versatile Engineered Nanomaterial Generation System (VENGES)”. Journal of Nanoparticle Research 14, 812 (811-817) (2012). link