Biological Analysis Service

Bachoo-9vv (Bachoo-9vv-vessels.jpg)The Biological Analysis Service provides expert advice and instrumentation support for Center biological research projects that analyze animals, tissues, cells or molecules. The service offers  access to and monetary support (in the form of Facility Access Funds) for both internal facilities and the many other important technologies available in the adjacent Harvard medical or Cambridge campus. The goal is to accelerate the productivity of NIEHS investigations using biological specimens and to facilitate use of new and powerful methodologies.

Services

◊  Flow Cytometry & Imaging (HSPH, Room II-231)  This laboratory houses a flow cytometer (BD Canto II analyzer), BD Pathway Scanning Cytometer, Tecan fluorescent plate reader, Molecular Devices Spectramax plate reader; access to SECTOR Imager 2400 reader for Meso-Scale System for multiplex assays is available.

Contact:
Flow Cytometry: Sally Bedugnis, 617-432-1277
Biomedical Imaging: Glen Deloid, 617-432-1277

◊  Molecular Analysis  (HSPH, Room II-231)   BioRad iCycler, Applied Biosysems 7300; Nanodrop for nucleic acid quantitation, and Alpha Innotech HD2 Chemiluminescence and Gel Imaging Station.

Contact: Lester Kobzik

◊  Pyrosequencing  (HSPH, B9)  Qiagen 96-well pyrosequencing station.

Contact: Kaylene Lin, 617-432-0478

◊  Scanning Electron Microscopy (Room II-210). This laboratory houses a Carl Zeiss 1450 VP Scanning Electron Microscope with Integrated Inca300/SEM Si(Li) X-ray detector and X-ray, INCA and ISIS analysis systems, and Leo-32 image analysis system, and important accessories such as the Critical Point Drier (CPD), (Tousimis, Smdri-PVT-3B) and Sputter Coater (Anatech, Hummer V).

Contact:  John Godleski, 617-432-1252

◊  Additional HSPH shared resources available to NIEHS investigators through institutional support at HSPH include: the SECTOR Imager 2400A (reader for Meso-Scale platform of multiplexed ELISA assays) and the HemavetTM 950FS Hematology Analyzer/CountessTM (rodent blood analysis in small volumes, SpectraMax M3 Multi-Mode Microplate Reader Workstations (available in Center for Population Health Research), and a Shared Qbiogene FastPrep FP120 Homogenizer (bead beater) for Cell Lysis. The Biological Analysis Service provides scheduling and training in use of these instruments.

◊  Off-Site Resources  See our listing of external facilities for imaging and biological analysis in the Harvard Medical Area and throughout Harvard.

How to Access Services

To use any of the services listed above, contact the specified facility manager, and follow these general guidelines

  • New users must be trained by the facility manager and be approved to use the equipment in the facility.
  • All users must obtain the financial approval from their PI and financial manager, and submit a PO number or the Harvard billing code prior to using the facility.
  • Approved users must sign up for equipment time with the facility manager.

Contact

For general information and to arrange consultation, contact:

Bioanalysis Service Director: Lester Kobzik
Bioanalysis Service Administrator: Marshall Katler