I. Genotyping Service
I.1. ABI Prism Taqman Allelic Discrimination Assay
Feature:
Applied Biosystems's (ABI) TaqmanÒ Assay is performed
using ABI Prism 7900 HT Sequence Detection System, which allows
single-plex SNP interrogation through fluorescence detection in
real-time PCR.
| Pricing |
| Set-up Fee: |
$250 per SNP (for < 700 DNA samples) |
| Taqman Genotyping Cost: |
$465 per SNP (for assays currently in stock)
$905 per SNP (for assays currently not in stock)
$0.60 per SNP per DNA sample (not DF/HCC)
$0.50 per SNP per DNA sample (DF/HCC discount)
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I.2. Biotrove SNP Genotyping Platform
Feature:
We now offer a new genotyping service from Biotrove. The
Biotrove OpenArray SNP Genotyping Platform allows researchers to run
16, 32 or 64 Taqman assays on one OpenArray chip. The OpenArray
chips require only 33nl of assay allowing for significant cost
savings.
| Pricing |
| Biotrove Genotyping Cost: |
As pricing varies according to the
number of samples and number of SNPs that are run, please
contact Hardeep Ranu for pricing information. |
Contact:
Hardeep K. Ranu PhD
Genotyping Manager
Program in Molecular and Genetic Epidemiology
Department of Epidemiology
Building II, Room 255
Harvard School of Public Health
655 Huntington Avenue
Boston, MA 02115
Phone: 617-432-4469
Fax: 617-432-1722
Email: hranu@hsph.harvard.edu
I.3. Quality
Assurance
For SNPs with
existing assays, please visit the following website.
For new SNP
assays, trial primer and assay design will be carried out and a
trial genotyping run performed. Depending on those results, we will
either proceed with full-scale genotyping or consider alternative
genotyping methods as necessary. Quality control procedures include
repeat genotyping of at least 5% of DNA samples. Genotype results
are provided to the user in electronic format. Extensive bioinformatics
support for our Core Facility is in place.
II. DNA
Extraction, Sample Handling, and Whole Genome Amplification Service
II.1. DNA
Extraction and Sample Handling
Feature:
DNA extraction is performed using QIAmp Blood Kit (Qiagen, Inc.).
We have been successful extracting DNA from WBCs, whole blood, blood
spots, residual red cell samples and buccal cells. We currently
use the Qiagen company protocol which yields high quality DNA in
200ul of buffer ranging from 5-50ng/ul. The concentration and plating
or tube storage is included in the cost. We have 3 robots that assist
in protocol set up and sample plating.
| Pricing |
| Extraction Cost: |
$11 per 50-200 ul WBCs, buccal cells, or whole blood (not DF/HCC)
$10 per 50-200 ul WBCs, buccal cells, or whole blood (DF/HCC discount) |
II.2. Whole
Genome Amplification
Feature:
Whole
genome amplification using the GenomiPhi DNA Amplification Kit from
Amersham Biosciences is used to amplify linear genomic DNA by Multiple
Displacement Amplification (MDA). This Kit is based on the high
processivity, high fidelity, and strand displacement ability of
bacteriophage Phi29 DNA polymerase. The typical yield is 3-7 micrograms
of amplified DNA from as little as 10-20 ng of genomic DNA template.
| Pricing |
| WGA Amplification Cost: |
$11 per sample (2 ul of 5 ng/ul)
for a single reaction (not DF/HCC)
$10 per sample (2 ul of 5 ng/ul) for a single reaction (DF/HCC discount) |
Contact:
Patrice
Soule
Lab Manager
Program in Molecular and Genetic Epidemiology
Department of Epidemiology
Building II, Room 226
Harvard School of Public Health
655 Huntington Avenue
Boston, MA 02115
Phone: 617-432-6101
Fax: 617-432-1722
Email: psoule@hsph.harvard.edu
Copyright, 2007, President and Fellows of Harvard College
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