Director, Harvard Proteomics Resource

Our lab is interested in developing and applying new technologies in the field of mass spectrometry-based proteomics.  We are particularly interested in methodologies for

a.) efficient sample preparation followed by proteomic analysis;

b.) sensitive, efficient and selective protein and peptide separation platforms; 

c.) quantitative characterization of alterations in protein abundance levels and post-translational protein modifications in response to environmental challenges;

d.) development of novel statistical and bioinformatics algorithms for data analysis;

e.) linking changes in proteomic profiles to system-wide changes for better understanding of molecular mechanisms underlying biology of cellular responses to environmental stimuli. 

These technologies are being developed, validated and demonstrated using the model organism Schizosaccharomyces pombe and cultured mammalian cells. 

Oxidative stress response is a topic of our pilot studies.  While mRNA profiling has begun to address functional aspects of a genome, several recent studies concluded that transcriptomic patterns are only a partial reflection of the functional state of an organism.  A comprehensive understanding of the genomic information will require a systems biology approach including information on quantitative differences in protein expression and protein modifications on a proteome-wide scale.