Purpose & Services Offered
The aims of the EGS are: to assist Center investigators in preparing genomic DNA from blood/buccal swabs collected in their human population studies. This activity involves assistance in the proper collection, extraction, concentration, quality testing and hand or robotic plating for candidate gene and even Genome-Wide studies of gene-environment interaction; to assist Center investigators in creating a genomic DNA biorepository for DNA for genotyping and for epigenetics studies; to assist investigators in obtaining efficient genotyping and epigenetic services either in-house or through partner organizations in the Harvard system; and to assist Center investigators in selection of SNP's, haplotypes, genotyping platforms and for environmental genetic and epigenetic studies of human populations.
Contacts
Please contact Li Su to engage the service of the facility.
Equipment
- ABI Taqman 7900HT Fast Real time PCR system
- Gentra AutoPure DNA extraction machine
- Promega Maxwell 16 Auto Tissue sample RNA /DNA extraction machine
- Spectra Max M5 384 -well DNA/RNA/protein/Elisa Quantitator (Pico Green)
- Beckman Coulter Biomek 2000 Laboratory Automation System (96/384 wells)
- 3 Geneamp PCR 9700 Machinces
- DNA Engine 2 x 384 well PCR Peltier Thermal Cyclers
- Pharmacia Biotech DNA/RNA Quantitainer
- Base Runner 200 Nucleic Acid Sequencer (Dual Capacity)
People
Director: David Christiani, Professor of Occupational Medicine and Epidemiology, Department of Environmental Health and Department of Epidemiology; Professor of Medicine, Harvard Medical School.
Administrator: John Yong
Service Coordinator: Li Su
FAQ
How do I engage the service?
To obtain services from either internal HSPH or external collaborating facilities, NIEHS Center investigators apply for facility use or funds by completing as simple, single page application that details the service, cost, and relevance to the NIEHS Center research mission. The form is submitted electronically to Ms. Li Su (Research Specialist and service coordinator) or Dr. David Christiani. The application is reviewed by Dr. Christiani, and two other Center Executive Committee members. If approved, Ms. Su arranges the appropriate billing codes for internal facilities or purchase orders for external facilities. She also maintains a central record of all applications, decisions, and funds dispensed that allows fiscal management and easy review of service utilization.
Examples