The HSPH-NIEHS Nanosafety Center is a member of the Nanotechnology Health Implications Research (NHIR) consortium, which focuses on nanosafety research, and was established by the National Institute of Environmental Health Sciences (NIEHS). The center brings together researchers from two of Harvard University’s schools (T.H. Chan School of Public Health [HSPH] and School of Engineering and Applied Sciences [SEAS]), Massachusetts Institute of Technology (MIT), University of Maine (UM) and University of Florida (UF). The HSPH-NIEHS Nanosafety Center builds upon the nano-related infrastructure in these collaborating universities, developed over the past 10 years, which includes an interdisciplinary research group of faculty, research staff, and students, as well as state-of-the-art platforms for high-throughput synthesis of ENMs, including metal and metal oxides, cutting edge2D/3D ENMs such as CNTs and graphene, nanocellulose, and advanced nanocomposites, coupled with innovative tools to assess the fate and transport of ENMs in biological systems, statistical and exposure assessment tools, and novel in vitro and in vivo platforms for nanotoxicology research.