Nurses’ Health Studies

If you follow nutrition news in the media, chances are good that you have come across findings from a cohort called the Nurses’ Health Study. The Nurses’ Health Study (NHS) began in 1976, spearheaded by researchers from the Channing Laboratory at the Brigham and Women’s Hospital, Harvard Medical School, and the Harvard T.H. Chan School of Public Health, with funding from the National Institutes of Health. It gathered registered nurses ages 30-55 years from across the U.S. to respond to a series of questionnaires. Nurses were specifically chosen because of their ability to complete the health-related, often very technical, questionnaires thoroughly and accurately. They showed motivation to participate in the long-term study that required ongoing questionnaires every two years. Furthermore, the group provided blood, urine, and other samples over the course of the study.

The NHS is a prospective cohort study, meaning a group of people who are followed forward in time to examine lifestyle habits or other characteristics to see if they develop a disease, death or some other indicated outcome. In comparison, a retrospective cohort study would specify a disease or outcome and look back in time at the group to see if there were common factors leading to the disease or outcome. A benefit of prospective studies over retrospective studies is greater accuracy in reporting details, such as food intake, that is not distorted by the diagnosis of illness.

To date, there are three NHS cohorts: NHS original cohort, NHS II, and NHS 3. Below are some features unique to each cohort.

NHS  – Original Cohort

  • Started in 1976 by Frank Speizer, M.D.
  • Participants: 121,700 married women, ages 30 to 55 in 1976.
  • Outcomes studied: Impact of contraceptive methods and smoking on breast cancer; later this was expanded to observe other lifestyle factors and behaviors in relation to 30 diseases.
  • A food frequency questionnaire was added in 1980 to collect information on dietary intake, and continues to be collected every four years.


  • Started in 1989 by Walter Willett, M.D., M.P.H., Dr.P.H., and colleagues.
  • Participants: 116,430 single and married women, ages 25 to 42 in 1989.
  • Outcomes studied: Impact on women’s health of oral contraceptives initiated during adolescence, diet and physical activity in adolescence, and lifestyle risk factors in a younger population than the NHS Original Cohort. The wide range of diseases examined in the original NHS is now also being studied in NHSII.
  • The first food frequency questionnaire was collected in 1991, and is collected every four years.


  • Started in 2010 by Jorge Chavarro, M.D., Sc.M., Sc.D, Walter Willett, M.D., M.P.H., Dr.P.H., Janet Rich-Edwards, Sc.D., M.P.H, and Stacey Missmer, Sc.D.
  • Participants: Expanded to include not just registered nurses but licensed practical nurses (LPN) and licensed vocational nurses (LVN), ages 19 to 46. Enrollment is currently open.
  • Inclusion of more diverse population of nurses, including male nurses and nurses from Canada.
  • Outcomes studied: Dietary patterns, lifestyle, environment, and nursing occupational exposures that may impact men’s and women’s health; the impact of new hormone preparations and fertility/pregnancy on women’s health; relationship of diet in adolescence on breast cancer risk.

From these three cohorts, extensive research has been published regarding the association of diet, smoking, physical activity levels, overweight and obesity, oral contraceptive use, hormone therapy, endogenous hormones, dietary factors, sleep, genetics and other behaviors and characteristics with various diseases. (1) In 2016, in celebration of the 40th Anniversary of NHS, the American Journal of Public Health’s September issue was dedicated to featuring the many contributions of the Nurses’ Health Studies to public health.

Growing Up Today Study (GUTS)

In 1996, recruitment began for a new cross-generational cohort called GUTS (Growing Up Today Study)—children of nurses from the NHS II. GUTS is composed of 27,802 girls and boys who were between the ages of 9 and 17 at the time of enrollment. As the entire cohort has entered adulthood, they complete annual questionnaires including information on dietary intake, weight changes, exercise level, substance and alcohol use, body image, and environmental factors. Researchers are looking at conditions more common in young adults such as asthma, skin cancer, eating disorders, and sports injuries.


  1. Colditz, G.A., Philpott, S.E., Hankinson, S.E. The Impact of the Nurses’ Health Study on Population Health: Prevention, Translation, and Control. American Journal of Public Health: Sept 2016, Vol. 106, No. 9, pp. 1540-1545.

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