Mark and Catherine Winkler Associate Professor of Environmental Epigenetics
Bldg 1, Rm G5, 665 Huntington Ave, Boston, MA 02115
Phone: (617) 432-0037; Fax: (617) 432-6913
Landmark Center, Suite 415 West,
P.O. Box 15677, 401 Park Dr, Boston, MA 02215
Phone: (617) 384-8742
My Laboratory of Human Environmental Epigenomics investigates the molecular mechanisms and biomarkers that reflect reprogramming of health and disease trajectories in response to environmental exposures. The laboratory research activities are specifically focused on epigenetics, environmental mitochondriomics, and computational epigenomics.
Epigenetic marks, including DNA methylation, histone modifications, and non-coding RNAs, modify chromatin structure and gene expression without changing the underlying DNA sequence. Unlike genetic mutations, which represent rare events with permanent consequences on genes, epigenetic changes are reversible and responsive to environmental influences. Using a highly quantitative Pyrosequencing-based approach and genome-scale microarray analyses for DNA methylation analysis, my laboratory has been examining responses of DNA methylation to a variety of environmental pollutants, including particulate air pollution, airborne benzene, metals, pesticides, dioxin-like compounds, and persistent organic pollutants, which are well known to be relevant to disease causation. We are dedicated to using state-of-art techniques for epigenomic analysis, not only investigating DNA methylation, but also histone modifications and expression of short non-coding miRNAs. We are currently testing novel methods for genome-wide epigenomic analyses based on next-generation sequencing.
Health Trajectories and Programming of Future Disease Risks
The epigenetic effects I have helped unveil can potentially modify health trajectories and affect disease risk. My laboratory has shown that epigenetic alterations similar to those induced by environmental exposures can be used to predict the risk of highly common human diseases, including cardiovascular disease, respiratory disease, and cancer. Ongoing projects include investigations of disease outcomes at different life stages, including fetal-growth restriction, childhood obesity, blood pressure and respiratory function, and age-related cognitive decline. My laboratory has been conducting studies on the U.S. population, as well as in highly-exposed groups or special conditions of exposure at several international locations in China, Canada, Mexico, Italy, Poland, Thailand, Oman, Bulgaria, Russia, and other countries.
Most of the epigenetic effects of environmental exposures that I have identified might be generated through oxidative stress. Not only mitochondria are a primary target of environmental oxidative damage, but most importantly damaged mitochondria become a main source of intra-cellular oxidation. Due to the paucity of repair mechanisms, mitochondrial DNA is expected to accumulate oxidative damage and thus provide a molecular archive of past environments and aggregate risk. Consistent with this hypothesis, we recently showed that air pollution and lead increase the blood abundance of mtDNAmolecules, a marker of damaged, dysfunctional mitochondrial DNA, by up to 50%. Following these exciting results, I have established a program of environmental mitochondriomics in my lab. We propose that mitochondria are uniquely sensitive to environmental toxics. If successful, our research in mitochondriomics will identify new non-invasive methods to reconstruct past exposures and identify individuals at risk of developing disease. Because of the central roles of oxidation and mitochondria in environmental causation of disease, mitochondriomics could provide models that can be applied to a variety of risk factors and health-related conditions.
Due to the recent explosion of datasets featuring epigenomics and molecular data, computational methods and quantitative genomics play an increasing role in providing effective approaches to analyzing and summarizing data. Computational tools are critical not only to directing the selection of key experiments, but also in formulating new testable hypotheses through detailed analysis of complex molecular information that is not achievable using traditional approaches alone. The Harvard School of Public Health (HSPH) is taking a leading role in interdisciplinary research involving the computational analysis of complex relationships between genes and their environment as well as basic biological and quantitative sciences. The laboratory is dynamically involved in the activity of the Computational Epigenomics Working Group (coordinated by Dr. Lin and Dr. Baccarelli), which is dedicated to developing and applying novel approaches for genome-scale epigenomic analysis. Recent activity included establishing a standardized enhanced pipeline for bioinformatic and biostatistical analysis of 450K Methylation BeadChip data, as well as for reduced representation bisulfite sequencing (RRBS) data.
Education and Training
1995 – M.D., University of Perugia, Italy
2000 – M.P.H., University of Turin, Italy
2003 – Ph.D., University of Milan, Italy
2000-2004 Post-Doctoral Fellowship, National Cancer Institute, Bethesda, MD