2024 PQG Conference

Ting Wu

Ting Wu, PhD
Professor of Genetics
Harvard Medical School

 

Chromosomes at super-resolution and a 300 million year old mystery

Our studies of genome organization have led us to develop tools for imaging the genome in situ using diffraction-limited light microscopy as well as single-molecule super-resolution microscopy. Here, I will summarize our progress with Oligopaints, OligoSTORM, and OligoDNA-PAINT. Time permitting, I will touch on SNP-resolved Hi-C to decipher the structure of paired homologs and the potential importance of somatic pairing in sequence ultraconservation.  

Nir, Guy (1); McCole, Ruth B (1), Erceg, Jelena (1); AlHaj Abed, Jumana (1); Beliveau, Brian J. (2,3); Sasaki, Hiroshi (2,3); Claire Redin (4,5,6); Golborodko, Anton (7,8); Lajoie, Bryan (9); Nguyen, Son C (1*); Saylor, Wren (1); Chamith Y Fonseka (1), Harrison Brand (4,5,6), Fudenberg, Geoffrey (7,8); Abdennur, Nezar (7,8); Imakaev, Maxim (7,8); Bintu, Bogdan (10,11); Hannan, Mohammed (1); Zhuang, Xiaowei (10,11,12); Yin, Peng (2,3); Lieberman Aiden, Erez (13); Dekker, Job (6,14); Mike Talkowski (4,5,6), Mirny, Leonid (4,5); Wu, Ting (C.-ting) (1) 

  1. Department of Genetics, Harvard Medical School, Boston, MA 02115
  2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115
  3. Department of Systems Biology, Harvard Medical School, Boston, MA 02115
  4. Center for Genomic Medicine, Massachusetts General Hospital and Harvard Medical School,     Boston, MA 02114, USA.
  5. Department of Neurology, Massachusetts General Hospital and Harvard Medical School,           Boston, MA 02114, USA.
  6. Stanley Center for Psychiatric Research and Program in Medical and Population Genetics, Broad Institute, Cambridge, MA 02141, USA.
  7. Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02138
  8. Institute for Medical Engineering and Science, Massachusetts Institute of     Technology, Cambridge, MA 02138
  9. Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605
  10. Department of Physics, Harvard University, Cambridge, MA 02138
  11. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138
  12. Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138
  13. Department of Genetics, Baylor College of Medicine, Houston, TX 77030
  14. Howard Hughes Medical Institute, University of Massachusetts, Worcester 01605